In remarks during talks with US Vice Mike Pence in Amman, the king said the only solution to the Israeli-Palestinian conflict was a two-state one.
Jordan lost East Jerusalem and the West Bank to Israel during the 1967 Arab-Israeli war.
US endorsement of Israels claim to Jerusalem as its capital broke with decades. US policy that the citys status must be decided in negotiations with the Palestinians, who want East Jerusalem as the capital of their future state.
King Abdullah said the US move would fuel radicalism and inflame Muslim and Christian tensions.
"For us, Jerusalem is key to Muslims and Christians, as it is to Jews. It is key to peace in the region and key to enabling Muslims to effectively fight some of our root causes of radicalisation," he said.